12 k a. oryzae oligonucleotide microarrays  (FermLab Ltd)

Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

Article Title: In silico Analysis of 3′-End-Processing Signals in Aspergillus oryzae Using Expressed Sequence Tags and Genomic Sequencing Data

doi: 10.1093/dnares/dsr011

Figure Lengend Snippet: Profile of the A. oryzae poly(A) data set. ( A ) Frequency distribution of EST contigs based on the EST copy number. The EST copy number of each contig contained in the A. oryzae poly(A) data set was obtained from the A. oryzae EST database ( http://nribf2.nrib.go.jp/EST2/index.html ). Data on the total EST contigs were obtained from the study by Akao et al . ( B ) Gene expression levels determined by DNA microarray analysis. The fluorescence intensity of each gene was normalized to that of the histone H4 gene.

Article Snippet: The A. oryzae wild-type strain RIB40, which was used for genome sequencing analysis, was grown in sterilized wheat bran media (3.0 g wheat bran with 1.8 ml distilled water) at 30°C for 33 h. Total RNA extraction, mRNA preparation, and DNA microarray analysis were performed according to the methods of Tamano et al . Purified Cy3- or Cy5-labelled cDNA probes were hybridized using 12 K A. oryzae oligonucleotide microarrays (Fermlab, Tokyo, Japan).

Techniques: Gene Expression, Microarray, Fluorescence

Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

Article Title: In silico Analysis of 3′-End-Processing Signals in Aspergillus oryzae Using Expressed Sequence Tags and Genomic Sequencing Data

doi: 10.1093/dnares/dsr011

Figure Lengend Snippet: A schematic representation of the alignment of 3′-end-processing signals in A. oryzae , yeast, and plants. The arrow indicates the cleavage and polyadenylation site.

Article Snippet: The A. oryzae wild-type strain RIB40, which was used for genome sequencing analysis, was grown in sterilized wheat bran media (3.0 g wheat bran with 1.8 ml distilled water) at 30°C for 33 h. Total RNA extraction, mRNA preparation, and DNA microarray analysis were performed according to the methods of Tamano et al . Purified Cy3- or Cy5-labelled cDNA probes were hybridized using 12 K A. oryzae oligonucleotide microarrays (Fermlab, Tokyo, Japan).

Techniques:

Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

Article Title: In silico Analysis of 3′-End-Processing Signals in Aspergillus oryzae Using Expressed Sequence Tags and Genomic Sequencing Data

doi: 10.1093/dnares/dsr011

Figure Lengend Snippet: Comparison of protein factors involved in pre-mRNA 3′-end-processing between Aspergillus oryzae , yeast, plants, and human

Article Snippet: The A. oryzae wild-type strain RIB40, which was used for genome sequencing analysis, was grown in sterilized wheat bran media (3.0 g wheat bran with 1.8 ml distilled water) at 30°C for 33 h. Total RNA extraction, mRNA preparation, and DNA microarray analysis were performed according to the methods of Tamano et al . Purified Cy3- or Cy5-labelled cDNA probes were hybridized using 12 K A. oryzae oligonucleotide microarrays (Fermlab, Tokyo, Japan).

Techniques: Comparison